Except as otherwise set forth in Sections II.D.2-4 below, to be timely, a Manager must exercise his challenge (by verbal communication to the appropriate Umpire), or the Crew Chief must initiate Replay Review (if applicable pursuant to Section II.C above) before the commencement of the next play or pitch. Such challenge or request will be considered timely only if the Umpire acknowledges that communication within the time period specified above. For purposes of these Regulations, the next "play" shall commence when the pitcher is on the rubber preparing to start his delivery and the batter has entered the batter's box (unless the defensive team initiates an appeal play in which case any call made during the play prior to the appeal still may be subject to Replay Review). A challenge to a play that ends the game must be invoked immediately upon the conclusion of the play, and both Clubs shall remain in their dugouts until the Replay Official issues his decision. No substitutions or pitching changes may take place while the Umpires are in the process of invoking Replay Review.